Saw it a second time last night. I had to make sure it was what it was when my brain wasn't melted from excitement. It's just as great on the second viewing.
Some big audience reactions people aren't mentioning much:
"I watched you while you were sleeping."
Thor uppercutting Hulk with Mjolnir made several people yell "Holy shit!"
"Son, you've got a condition"
And though most people had left the theater, the shawarma bit got a big round of applause and laughter both times.
A small thing: Cap balling up behind his shield before the Chitauri bomb went off inside that building was awesome. I missed that the first time.
Both audiences were incredibly quiet after Coulson's death. Credit to the filmmakers for giving that the poignancy it needed. The audiences seemed genuinely affected by it. I did not, however, like Fury lying about the Captain America cards in Coulson's jacket. I felt like it diminished his death a little bit.
I really like the kinship established between Banner and Stark. I hope Ruffalo has a role in IM3. He's really good as Banner.
Also, virtually nobody got the Point Break line in either show I went to. That seems to be the most obscure reference in the film and it's not even that obscure I don't think. In comparison, everybody got the Stephen Hawking bit between Rogers and Coulson, which I didn't expect.
Also, did I detect seeds of romance between Natasha and Banner or is that a reach? It seems like it's barely there, but the look on her face when he transforms in the helicarrier, and then her passing him his bag in the ending (they were riding together?) made me think something was there.
Oh, and the "courting death" line to Thanos at the end was a very nice touch. I didn't make the connection the first time.