I grew out of 1up Yours a long time ago. Sometimes it just feels like a professional forum troll flamewar in podcast form, and, at least back when I stopped listening to it, it went on ridiculously long (three hours?!). Too much angry debating and too much childish sniping for my tastes.
I started listening to On The Spot 'cause Jeff and the gang were a lot less cynical and bitchy, but they were still funny as hell. It was just more fun to listen to overall - it wasn't some alcoholic sailors screaming at each other over business politics, it was just friends having fun. I followed Jeff to Giant Bomb and I'm overjoyed to hear some of my favorite guys from Gamespot (and On The Spot) are on the team.
A+ would listen again.