Okay guys, I've compiled what I believe to be a complete list of "one-and-done" games for NES, Genesis, and SNES, based on their North American libraries, with games listed according to the number of GameFAQs user votes they've got. May be of interest to some people, so I'll post them. I've put asterisks beside borderline games, which you may count or discount at your pleasure.
NES: 150 games out of 758
The Adventures of Bayou Billy- 231
Abadox- 176
Dr. Jekyll and Mr. Hyde- 162
Gyromite- 136
Deadly Towers- 135
Metal Storm- 133
Monster Party- 131
8 Eyes- 127
Clash at Demonhead- 122
Cobra Triangle- 121
Shatterhand*- 116 [JP version is based on Super Rescue Solbrain]
Color a Dinosaur- 111
Kabuki Quantum Fighter- 111
Anticipation- 106
Wrath of the Black Manta- 101
Xexyz- 97
Demon Sword- 95
Code Name: Viper- 94
Vice: Project Doom- 94
Adventures of Dino Riki- 93
Air Fortress- 93
Little Samson- 91
The Magic of Scheherazade- 89
Swords and Serpents- 80
Amagon-77
Kickmaster- 76
Gun Nac- 74
Base Wars- 73
Conquest of the Crystal Palace- 73
Werewolf: The Last Warrior- 71
Othello*- 67 [not clear if this is based on a licensed version of the game]
Time Lord- 66
Iron Tank*- 61 [sort-of sequel to TNK III]
Tecmo World Wrestling- 60
Low G Man- 59
Nightshade- 55
Adventures of Tom Sawyer- 54
Totally Rad- 54
Dynowarz: Destruction of Sponylus- 52
Taboo: The Sixth Sense- 52
Arkista’s Ring- 51
Ninja Crusaders- 48
Panic Restaurant- 48
Bad News Baseball*- 47
The Adventures of Rad Gravity- 43
To the Earth- 43
Sword Master- 42 [quasi-sequel to Castle of Dragon]
Pinball Quest- 41
Kid Kool*- 40
Star Voyager- 40
Dash Galaxy in the Alien Asylum- 38
Quattro Adventure*- 38 [only 1 of the 4 games qualifies]
Battle Tank- 37
Dragon Fighter- 37
Frankenstein: The Monster Returns- 37
Isolated Warrior- 37
Vegas Dream- 37
Desert Commander- 35
Eliminator Boat Duel- 35
Bible Buffet- 34
Ghoul School- 34
Day Dreamin’ Davey- 33
Ghost Lion- 33
Zombie Nation- 33
Dungeon Magic: Sword of the Elements- 32
Wurm: Journey to the Center of the Earth- 32
Stealth ATF- 31
Faria- 30
Chubby Cherub*- 28 [JP version is based on Q-taro]
Digger T. Rock- 28
Master Chu and the Drunkard Hu- 28
Metal Mech: Man & Machine- 28
Super Glove Ball- 28
The King of Kings: The Early Years- 27
Circus Caper- 26
Dusty Diamond’s All-Star Softball- 26
The Ultimate Stuntman- 26
Galaxy 5000: Racing in the 51st Century- 25
Stack-Up- 25
Formula One: Built to Win- 24
Krazy Kreatures- 24
Destination Earthstar- 23
Phantom Fighter*- 23 [JP version is based on the movie Mr. Vampire]
Magician- 22
Treasure Master- 22
Defenders of Dynatron City- 21
Little League Baseball: Championship Series*- 21 [sort-of spinoff to Baseball Stars]
All-Pro Basketball- 20
Monster Truck Rally- 20
Starship Hector*- 20
Baby Boomer- 19
Bandai Golf: Challenge Pebble Beach*- 19 [not sure if this game is licensed or not]
Space Shuttle Project- 19
Ultimate Air Combat- 19
Bee 52- 18
Capcom’s Gold Medal Challenge ’92- 18
Cowboy Kid- 18
Laser Invasion-18
Sqoon*- 18 [Received a quasi-sequel, In the Hunt, on the Neo Geo]
Tagin’ Dragon- 18
Crystal Mines- 17
Dudes with Attitude- 17
F-117A Stealth Fighter- 17
Peek A Boo Poker- 17
Race America*- 17 [European version is a Chevrolet-licensed game]
Big Nose Freaks Out- 16
Orb 3D- 16
Deathbots- 15
F-15 City War- 15
Heavy Shreddin’- 15
Mission Cobra- 15
Pyramid- 15
Magic Darts- 14
Raid 2020- 14
Shooting Range- 14
Blackjack- 13
Challenge of the Dragon- 13
King Neptune’s Adventure- 13
Puzzle- 13
Videomation- 13
Metal Fighter- 12
Operation Secret Storm- 12
Quattro Arcade*- 12 [only 2 of the 4 games qualify]
Silent Assault- 12
World Champ- 12
Castle of Deceit- 11
Death Race- 11
Galactic Crusader- 11
Motor City Patrol- 11
The Mutant Virus: Crisis in a Computer World- 11
Power Punch II- 11
Robodemons- 11
Rock ‘n Ball- 11
Break Time: The National Pool Tour- 10
Double Strike- 10
Secret Scout in the Temple of Demise- 10
Moon Ranger- 9
Solitaire- 9
Tiles of Fate- 9
Ultimate Basketball- 9
Golf Grand Slam- 7
P’radikus Conflict- 7
Short Order / Eggsplode!- 7
Stanley: The Search for Dr. Livingstone- 7
Stunt Kids- 7
Venice Beach Volleyball- 7
Rad Racket: Deluxe Tennis II- 5
Roundball 2-on-2 Challenge- 5
Shockwave- 5
Ultimate League Soccer- 5
Sega Genesis: 50 games out of 708
Crusader of Centy- 129
Alisia Dragoon- 122
Ranger X- 105
General Chaos- 104
Skitchin’*- 71 [sort-of spinoff of Road Rash]
Cyborg Justice- 67
Elemental Master- 67
Zero Tolerance- 62
Sub-Terrania- 61
Blades of Vengeance- 59
Gaiares- 59
Arrow Flash- 54
Jewel Master- 46
Awesome Possum- 36
Rolo to the Rescue- 34
F022 Interceptor- 30
Rings of Power- 28
Generations Lost- 27
Combat Cars- 26
Red Zone- 26
Traysia- 26
Fighting Masters- 25
Crystal’s Pony Tale- 24
Sorcerer’s Kingdom- 24
Tinhead- 24
Mystical Fighter- 22
Shove It! The Warehouse Game- 20
Whip Rush- 18
F-117 Night Storm- 17
Menacer 6-Game Cartridge- 17
Technoclash- 17
Beast Wrestler- 16
Bimini Run- 14
Gadget Twins- 14
Socket- 13
Death Duel- 12
Nightmare Circus- 12 [cartridge game in some places, Sega Channel-only in others; this variance may disqualify it]
Saint Sword- 12
Dino Land- 11
Shadow Blasters- 11
Trampoline Terror- 11
Ka-Ge-Ki: Fists of Steel- 9
Dashin’ Desperadoes*- 8 [Wikipedia claims it got a sequel on PS2, but I've found no info on it at all]
Earth Defense- 8
King Salmon: The Big Catch- 8
Quad Challenge- 8
Normy’s Beach Babe-O-Rama- 5
Mallet Legend’s Whac-A-Critter- 4
Pro Moves Soccer- 2
Funny World & Balloon Boy- 1
SNES: 77 games out of 720
Illusion of Gaia*- 944 [arguably affiliated with Soul Blazer/Terranigma/Granstream Saga]
Secret of Evermore*- 854 [marketed as a sort-of sequel to Secret of Mana]
Soul Blazer*- 443 [arguably affiliated with Illusion of Gaia/Terranigma/Granstream Saga]
The 7th Saga*- 310 [Mystic Ark is a quasi-sequel to this game]
Uniracers- 214
Stunt Race FX- 202
Robotrek- 180
Brain Lord- 160
Arcana- 134
Super Scope 6- 134
Skyblazer- 107
Metal Warriors*- 99 [Marketed as a sort-of follow-up to Cybernator]
Plok- 98
Tecmo Secret of the Stars- 96
Hagane- 82
Claymates*- 79 [Clayfighter was a sort-of follow-up to this game]
Lester the Unlikely- 71
Young Merlin- 59
King Arthur’s World- 54
Hyperzone- 52
Run Saber- 50
X-Kaliber 2097- 48
Ardy Lightfoot- 47
Xardion- 45
Nosferatu- 43
Tin Star- 39
Smart Ball- 36
Tuff E Nuff- 35
Vortex- 33
D-Force- 32
Big Sky Trooper- 30
Dirt Trax FX- 29
ASP: Air Strike Patrol- 28
Battle Cars- 28
Captain Novolin*- 27 [arguably affiliated with Rex Ronan/Bronky/Packy & Marlon]
Rocky Rodent- 26
Super Pinball: Behind the Mask*- 26
Extra Innings- 25
Firestriker- 25
HAL’s Hole in One Golf*- 25 [JP version features Jumbo Ozaki]
Musya- 25
Cacoma Knight in Bizyland- 24
Harley’s Humongous Adventure- 24
Time Slip- 19
Imperium- 18
Bazooka Blitzkrieg- 17
Cyber Spin*- 16 [JP version is based on Future GPX Cyber Formula]
Power Piggs of the Dark Age- 16
Dig & Spike Volleyball- 15
Pieces- 15
World Soccer ’94: Road to Glory- 15
Carrier Aces- 14
Doomsday Warrior- 14
Frantic Flea- 14
Hammerlock Wrestling*- 14 [JP version features Genichiro Tenryu]
Incantation- 14
Skuljagger: Revolt of the Westicans- 13
RPM Racing*- 12
Shien’s Revenge- 12
X-Zone- 12
Battle Grand Prix- 11
Realm- 11
Air Cavalry- 10
Metal Morph- 10
Mohawk and Headphone Jack- 10
Bronkie the Bronichiasaurus- 9 [arguably affiliated with Packy & Marlon/Rex Ronan/Captain Novolin]
Full Throttle All-America Racing- 9
Rex Ronan: Experimental Surgeon*- 9 [arguably affiliated with Packy & Marlon/Bronky/Captain Novolin]
Super Slap Shot*- 9 [apparently endorsed by Gordie Howe, not clear if it actually features him though]
International Tennis Tour- 8
Dream TV- 7
Space Football- 6
Mecarobot Golf*- 5 [JP version features Nobuo Serizawa
Street Hockey ’95- 5
Packy & Marlon*- 4 [arguably affiliated with Rex Ronan/Bronky/Captain Novolin]
Football Fury- 3
TKO Super Championship Boxing*- 5 [JP version features Jiro Matsushima on the cover]