Yeah, I wasn't sure if you were looking for any kind of advice or criticism because you said the trailer was complete. Since I have your permission, I'm going to offer you my thoughts.
The first seconds are everything in a Kickstarter pitch video. We have to wait through 20 seconds of cutscene and voiceover, which are not as impressive as the game itself and just generally not impressive, before we actually see the game. If I was browsing this listing there's a fair chance I wouldn't make it past those 20 seconds. I'd consider cutting those elements from the video were it me and just focus on the game, but if you feel you need to utilize them, then I'd cut them into the gameplay footage sporadically.
BUPs are a hard sell. There is a segment of people who are nostalgic for this type of thing, but broadly speaking I think this is an uphill battle from the get go. Modern BUPs that have been successful have rather involved RPG elements (Castle Crashers, Dragon's Crown). You do touch on this just a bit in the trailer (we see the beginnings of a fetch quest) but if it were my game and my project on the line I'd add a lot more emphasis onto these elements and mechanics in the pitch video. I wouldn't make it an afterthought or a sidenote. The RPG aspects (items, quests, levels, whatever) should be prominent in the video. If you have to mock up additional stuff to make this happen I would think it's probably worth it.
The pace and motion of the game is a bit stilted. It may not be realistic to rework sprite animations for the trailer (believe me, I know) but if funded I'd definitely consider devoting resources to improving the key poses of the various fighting moves. The game looks good at a glance, but I feel the poses and motions are unnatural and too slow. It may be possible to make things a bit more exciting with post editing (maybe some tweaks to the playback speed here and there, screen shakes, whatever) but largely I don't think this concern can be addressed without reworking some of the game itself, which I understand is probably not very realistic at this point.
I wish you the best of luck, I have concerns that this is an uphill battle to begin with (as I said), but it has a strong sense of identity, which is a good start. Many games don't have much of that. You're just appealing to a dwindling playerbase with 80s/90s beatemup nostalgia -- Little has been done in modern times to keep that genre alive.